Bacteria+Lab

=TITLE: __Does Sanatation Effect Small Kingdom Growth__=

=BACKGROUND:=


 * ==PURPOSE: //To see if sanatation effects small kindom growth.//==


 * ==HYPOTHESIS: We think that small kingdoms will grow more on the unsanitized side of the Petri dish than the sanitized side.==


 * ==VARIABLES: //The independent variable is does the sanitizer work?//==

The dependent is how much does the bacteria grow?- 1 percent coverage- 2 Type of bacteria

 * The controle is the temperature, they are all done on geloton, and the sanitizer is the same brand**

=MATERIALS:= These are the Materials Needed:
 * Petri Dish
 * Knox Gelatin (With Added Sugar)
 * 2 Opened Swabs
 * Purell Hand Sanitizer
 * Marker
 * Grid Transparency Paper
 * Dissecting Microscope or a Magnifying Glass
 * Para film Wax Tape

=PROCEDURES:= For the sanitizing experiment (after 24 hours of gelatin hardening) 1. Be sure you have used permanent marker to divide the Petri dish into four areas. One area should be labeled sanitized, a second area labeled unsanitized and a third area labeled control the last section should be blank. These labels need to be small but clear so that you can still see growth without having to remove the cover. 2. Use a clean swab to swipe the palm of one hand and between the fingers. 3. Gently remove the lid of the Petri dish and wipe the swab over the gelatin on the unsanitized side. Replace the lid and the tape. 4. Sanitize the hand with hand sanitizer. 5. Take a different swab and swipe the palm and fingers of the sanitized hand. 6. Gently remove the lid of the Petri dish and wipe the swab over the gelatin on the sanitized side. Replace the lid. 7. Allow the dish to sit for 4 days for the small kingdoms to grow. 8. Cut a grid to fit over the Petri dish, **WITHOUT** opening the lid to the dish. Using the grid, determine the percent coverage of the growth on both the sanitized side and the unsanitized side. Also, record the number of different growths.

=RESULTS:= Small Kingdom Growth
 * Table:**
 * Group || Control || Control || Not Sanitized || Not Sanitized || Sanitized || Sanitized ||
 * || %covered || number of types || %covered || number of types || %covered || number of types ||
 * Robert/Danielle || 16% || 3 || 41% || 3 || 3% || 1 ||
 * Ebby/Tim || 28% || 6 || 48% || 5 || 15% || 10 ||
 * Sonia/Anna || 51% || 2 || 12% || 1 || 9% || 1 ||
 * Derek/Jean || 29.7% || 2 || 33.3% || 1 || 23.8% || 1 ||
 * Ayonna/Danny || 91% || 4 || 21% || 3 || 80% || 5 ||
 * Average || 33.96% || 3.4 || 31.06% || 2.6 || 26.16% || 5.4 ||


 * Graph:**



//(Tim type graph)// =CONCLUSION:=

Our data does not show us if our hypothosis was correct because our control had the highest count of small kingdoms growth. This could have been because we did not proprely sanitized our materials like gelatin and our swabs.Suggestions for Future incluede:have the teacher in the room and give directions just before you do the experiment so that if anyone doesn't understauor contnd any of the directions, the teacher can help give them a more simpler version of them. Also, You should probally have everyone use a very strong permanent marker on the petri dishes, because they can wear away easily over a period of time. We should also have used a closed, new swab box. Because the control had more growth than sanitized and not sanitized meaning the swab was contaminated.